Dr. Interwebz was a mad scientist who created the human version of YouTube in the crossover episode, YouTube Meets Daneboe and The Annoying Orange!. He didn't appear for the remainder of the video, however his invention didn't go very well.
| Main Characters | Recurring Characters | TV Exclusive Characters |
|---|---|---|
| Orange | Apple | Nerville |
| Pear | Squash | Apple |
| Midget Apple | Knife | Coconut |
| Marshmallow | Liam the Leprechaun | Peach |
| Passion Fruit | Captain Obvious | Tomato |
| Grapefruit | Nude Dude | Banana |
| Grandpa Lemon | Dr. Bananas | Pineapple |
| Orange's Sister | Zoom | Ginger |
| Corey | Gaming Grape | Broccoli Overlord |